Heat stress induces phage tolerance in <i>Enterobacteriaceae</i>.

ABSTRACT: Antibiotic resistance and tolerance present significant challenges in global healthcare, necessitating alternative strategies such as phage therapy. However, the rapid emergence of phage-resistant mutants poses a potential risk. Here, using Klebsiella pneumoniae ATCC 43816 and its lytic phage Kp11 as a model system, we investigated bacterial persistence against phages, characterized by heterogeneous survival, analogous to antibiotic persistence. We found that heat treatment enhanced persistence and increased bacterial survival under phage exposure, subsequently promoting the evolution of phage resistance. Further experiments demonstrated that heat stress leads to a reduction in envelope components, thereby inhibiting phage DNA injection. Additionally, this heat-induced reduction res

SUBMITTER: Zhang F 

PROVIDER: S-EPMC12234005 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature