Comprehensive immune profiling and predictive modelling of paediatric acute hepatitis of unknown aetiology from a Spanish cohort.

ABSTRACT:

Introduction

Paediatric acute hepatitis of unknown aetiology (PAHUA) has emerged as a global health concern, yet its cause remains unidentified. This study characterises the clinical and immunological profiles of PAHUA to identify reliable immune biomarkers for accurate diagnosis.

Methods

Samples from 24 PAHUA patients, 6 children with autoimmune hepatitis (AIH), and 13 healthy paediatric volunteers (HVs) were analysed. Immunophenotyping, soluble immune checkpoints (ICs) and cytokine profiling, and ex vivo immune responses were assessed using spectral flow cytometry. Clustering and logistic regression modelling were used to identify predictive biomarkers.

Results

PAHUA cases frequently presented with gastrointestinal symptoms and liver damage preceding jaundice

SUBMITTER: Lozano-Rodriguez R 

PROVIDER: S-EPMC12235265 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature