Effects of dietary NDF/NFC ratios on <i>in vitro</i> rumen fermentation, methane emission, and microbial community composition.

ABSTRACT: The objective of this study was to investigate the effects of dietary neutral detergent fiber (NDF) to non-fibrous carbohydrate (NFC) ratios on in vitro rumen fermentation, methane production, and microbiota in dairy cows. In vitro rumen fermentation was conducted with five dietary groups: R0.48 (NDF/NFC = 0.48), R0.57 (NDF/NFC = 0.57), R0.70 (NDF/NFC = 0.70), R0.90 (NDF/NFC = 0.90), and R1.12 (NDF/NFC = 1.12). As the NDF/NFC ratios increased, total gas production decreased linearly. The degradation rates of dry matter (DM), neutral detergent fiber (NDF), and acid detergent fiber (ADF) decreased, showing a quadratic response (p = 0.018). Methane production per unit of DM was not significantly affected (p > 0.05), whereas methane production per unit of degraded D

SUBMITTER: Li J 

PROVIDER: S-EPMC12235747 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature