Systematic pairwise co-cultures uncover predominant negative interactions among human gut bacteria.

ABSTRACT:

Background

Understanding pairwise bacterial interactions in the human gut is crucial for deciphering the complex networks of bacterial interactions and their contributions to host health. However, there is a lack of large-scale experiments focusing on bacterial interactions within the human gut microbiome.

Methods

We investigated the pairwise interactions of 113 bacterial strains isolated from healthy Chinese volunteers, selected for their high abundance and functional representation of the human gut microbiome. Using mGAM agar plates, a rich medium designed to maintain community structure, we established the "PairInteraX" dataset, which includes 3233 pair combinations of culturable human gut bacteria. This dataset was analyzed to identify interaction patterns and the key fa

SUBMITTER: Zhu J 

PROVIDER: S-EPMC12235815 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature