Quantitative Thermodynamic Characterization of Self-Assembling RNA Nanostructures.

ABSTRACT: Recent developments in RNA nanotechnology have led to the rise in designing specific higher order RNA structures with functional goals in mind, such as drug delivery and immunomodulation. As researchers create RNA nanostructures with the goal of becoming common in the molecular biologists' toolkit, more investigation is required on the robustness of RNA designs. Primarily, what different molecular contexts are the designed and intended nanostructures stable in? In this work we show that by using second-order right-angle light scattering, that RNA nanostructure self-assembly is highly sensitive to environmental conditions. While a test RNA hexagonal grid nanostructure that forms correctly though 120° kissing loops under ideal conditions, small variations in salt conditions and annealing tim

SUBMITTER: Aposhian J 

PROVIDER: S-EPMC12236615 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature