Spatial joint profiling of DNA methylome and transcriptome in mammalian tissues.

ABSTRACT: The spatial resolution of omics dynamics is fundamental to understanding tissue biology. Spatial profiling of DNA methylation, which is a canonical epigenetic mark extensively implicated in transcriptional regulation, remains an unmet demand. Here, we introduce a method for whole genome spatial co-profiling of DNA methylation and transcriptome of the same tissue section at near single-cell resolution. Applying this technology to mouse embryogenesis and postnatal brain resulted in rich DNA-RNA bimodal tissue maps. These maps revealed the spatial context of known methylation biology and its interplay with gene expression. The two modalities' concordance and distinction in spatial patterns highlighted a synergistic molecular definition of cell identity in spatial programming of mammalian deve

SUBMITTER: Lee CN 

PROVIDER: S-EPMC12236673 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature