Global analysis of membrane protein S-acylation in the model plant <i>Arabidopsis thaliana</i>.

ABSTRACT: Protein S-acylation is the addition of fatty acids to the cysteine residues in a protein, catalyzed by protein S-acyltransferases (PATs). Despite extensive research on protein S-acylation in animals, our understanding of this process in plants remains limited. In this study, we sought to characterize the S-acylproteome of membrane proteins in Arabidopsis and identify potential substrates for two important plant immunity-related PATs (PAT5 and PAT9). To achieve this, S-acylated membrane proteins were first enriched via our optimized acyl-biotinyl exchange strategies at both the protein-level and peptide-level. The enriched samples were then analyzed by label-free quantitative liquid chromatography-mass spectrometry. The results from the two enrichment methods demonstrated that they w

SUBMITTER: Zhou L 

PROVIDER: S-EPMC12236689 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature