Sealable capped nanovials for high-throughput screening of cell growth and function.

ABSTRACT: Millions of modular nanoliter-scale compartments that isolate functionally rich single-cell and cell-to-cell communication data can scale biological discovery for the age of AI. Here, we introduce capped nanovials-suspendable, sealable microscale compartments formed by the docking of hydrogel capping particles into bowl-shaped nanovials-as a versatile system for culturing, analyzing, and sorting single cells and small colonies. This two-particle architecture enables localized confinement of cells and secreted products while maintaining compatibility with standard laboratory workflows such as wash and reagent exchange steps, fluorescence microscopy, and flow cytometry. Crucially, these compartments are formed via simple pipetting and centrifugation steps, making the platform highly democrat

SUBMITTER: Mellody M 

PROVIDER: S-EPMC12236721 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature