A mitotic bookmark coordinates transcription and replication.

ABSTRACT: Collisions between advancing replication forks and elongating transcripts pose a universal threat. During the rapid nuclear division cycles in early Drosophila embryos, coordinating transcription and replication is critical to reduce the risk of collisions. In each cycle, replication begins immediately after mitosis, while transcription starts 3 minutes later, overlapping with replication for the remainder of interphase. We previously showed that transcription depends on the coactivator Brd4, which forms hubs at active genes. Here, we show that Brd4 persists on mitotic chromosomes as bookmarks of transcriptional activity and, upon anaphase entry, recruits the replication activator Cdc7 to specify early-replicating genomic regions in the following interphase. Additionally, Cdc7 activ

SUBMITTER: Cho CY 

PROVIDER: S-EPMC12236780 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature