Thienopyrimidine amide analogs target MmpL3 in <i>Mycobacterium tuberculosis</i>.

ABSTRACT:

Objectives

The identification of novel agents with mechanisms of action distinct from those currently utilized in tuberculosis treatment remains a significant challenge. The mycobacterial protein MmpL3 has emerged as a promising drug target due to its essential role in the synthesis of the cell wall of Mycobacterium tuberculosis. We previously identified novel thienopyrimidine amides with good anti-tubercular activity.

Methods

We profiled a subset of thienopyrimidine amides determining activity against intracellular bacteria and bactericidal activity against replicating bacteria. We ran assays to determine mode of action by measuring cell wall stress, ATP production, and bacterial cytological profiling. We determined activity against a strain of M. tuberculosis with m

SUBMITTER: Baldin VP 

PROVIDER: S-EPMC12236842 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature