Exploration of the role and mechanism of Rhizoma Paridis total saponins in osteosarcoma based on SPI1/LCN2-mediated ferroptosis.

ABSTRACT:

Background

Osteosarcoma (OS) is a highly aggressive bone malignancy with limited therapeutic options and poor prognosis, particularly in cases of recurrence or metastasis. Recent studies have identified ferroptosis as a promising therapeutic target in OS, with the SPI1/LCN2 regulatory axis emerging as a critical modulator of this process. We hypothesized that Rhizoma Paridis total saponins (RPTS) exert anti-osteosarcoma effects by inducing ferroptosis through inhibition of the SPI1/LCN2 axis.

Methods

In vitro assessments using OS cell lines MG-63 and Saos-2 included cell counting kit-8 (CCK-8) assays for viability, colony formation for proliferation, scratch wound healing for migration, and Transwell chambers for invasion. Ferroptosis markers were evaluated using colo

SUBMITTER: Yang G 

PROVIDER: S-EPMC12237685 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature