Determination of residual DNA in decellularised aortas- towards fluorescence-based quantification of DNA purified by various methods.

ABSTRACT:

Background

Decellularisation of animal tissues is a promising strategy to obtain scaffolds for tissue engineering. However, double-stranded (ds)DNA of animal origin that may reside in tissue even after harsh decellularisation causes adverse reactions in patients. Thus, precise determination of residual dsDNA is essential, but challenging since the methods used for purification may affect quantification.

Methods

To elucidate the best method for purifying and quantifying residual dsDNA in decellularised vessels, rat thoracic aortas were perfused with detergents (sodium dodecyl sulfate/sodium deoxycholate) with or without DNase I. Native aortas were used as control. Three different methods for purifying DNA (tissue lysate, salting out, silica-based solid-phase) were applied to

SUBMITTER: Pentzold S 

PROVIDER: S-EPMC12238089 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature