Bacillus spore showcasing key antigens of African swine fever potentializes a promising oral vaccine candidate.

ABSTRACT: African swine fever (ASF) remains a widespread and fatal infectious disease affecting both domestic pigs and wild boars around the world, and there is still no vaccine available for full protection. In recent years, Bacillus spores have been developed into effective vaccine carriers. Here, Bacillus amyloliquefaciens spores from strain CPLK1314 were used as a vaccine delivery system to display three highly antigenic proteins, p72, p30, and p54 of ASF, and their immune responses in BALB/c mice were evaluated. Our results demonstrated that the antibiotic-free Bacillus gene editing system constructed in this study can work effectively, and p72 and the fusion antigen p3054 were successfully expressed in the spore coat of CPLK1314 by fusing with CotB and CotG, respectively. Further animal experi

SUBMITTER: Wang X 

PROVIDER: S-EPMC12238207 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature