SMAD2 linker phosphorylation impacts overall survival, proliferation, TGFβ1-dependent gene expression and pluripotency-related proteins in NSCLC.

ABSTRACT:

Background

We investigated the impact of SMAD2 linker phosphorylation (pSMAD2L) on overall and disease-free survival, signal transduction, as well as cancer-related processes in non-small cell lung cancer (NSCLC).

Methods

We generated A549 cells constitutively lacking pSMAD2L (A549L^sub) to gain mechanistic insights and stimulated NSCLC cell lines with inhibitors against cell cycle-associated kinases or TGFβ1. In addition, we analysed SMAD2 and pSMAD2L in alveolar epithelial cells type 2 from tumour-free lung tissue as well as in benign and malignant T cells by Western blotting. Furthermore, pSMAD2L-positive tumours and immune cells were analysed in an NSCLC patient cohort (n = 316) using multiplex immunofluorescence.

Results

In NSCLC cell lines and benign T

SUBMITTER: Nitschkowski D 

PROVIDER: S-EPMC12238225 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature