Heterotrimeric G-protein subunits regulate plant architecture, pod development, seed size, and symbiotic nodulation in <i>Medicago truncatula</i>.

ABSTRACT: Heterotrimeric G proteins are crucial transducers of signaling from receptors, participating in growth and development, as well as in responses to biotic and abiotic stimuli. However, little is known about their roles in regulating various yield-related traits in legumes. In this study, we systematically analyzed the functions of two G-protein-encoding genes, MtGα1 and MtGβ1, along with Regulator of G-protein Signaling1 (MtRGS1), in Medicago truncatula. All three genes were ubiquitously expressed in roots, stems, leaves, nodules, flowers, and pods. We generated the knockout mutants Mtgα1, Mtgβ1, and Mtrgs1 using CRISPR/Cas9 and assessed their growth and development. MtGα1 knockout resulted in slightly shorter plants with smalle

SUBMITTER: Sun F 

PROVIDER: S-EPMC12238709 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature