A rotavirus VP4 or VP7 monoreassortant panel identifies genotypes that are less susceptible to neutralization by systemic antibodies induced by vaccination or natural infection.

ABSTRACT: Rotavirus infections remain a significant cause of morbidity and mortality in infants. The viral surface proteins VP4 and VP7 are each classified into multiple genotypes (P[1]-P[58] for VP4 and G1-G42 for VP7), which differ in their susceptibility to neutralizing antibodies; however, detailed analyses of these differences remain limited. This study investigates the susceptibility of diverse VP4 and VP7 genotypes to neutralizing antibodies induced by vaccination or natural infection. A reverse genetics system based on the human rotavirus Odelia strain (G4P[8]) was used to create monoreassortant viruses by replacing the VP4 or VP7 genes with those from clinical isolates (obtained in Thailand) or prototype strains. We generated 11 VP4 and 19 VP7 reassortants, covering 7 and 17 genotypes, resp

SUBMITTER: Kotaki T 

PROVIDER: S-EPMC12239578 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature