Development and preclinical characterization of AMG 329: a human antibody neutralizing FLT3 ligand.

ABSTRACT: The feline McDonough sarcoma-like tyrosine kinase 3 (FLT3)/FLT3 ligand (FLT3L) signaling pathway regulates the development and activity of dendritic cells (DCs) and other myeloid cells, including monocytes. FLT3L, DCs, and monocytes have been implicated in several autoimmune diseases. Here, we describe the development and characterization of a human immunoglobulin G1λ monoclonal antibody (AMG 329; formerly MEDI1116/VIB-1116/HZN-1116) targeting human FLT3L. AMG 329 was derived from a large human combined antibody display library; it was optimized to enhance affinity for FLT3L and reduce antibody dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity. Binding affinity was determined by surface plasmon resonance interaction analysis. Specificity of FLT3L was measured usi

SUBMITTER: Lau-Kilby A 

PROVIDER: S-EPMC12239813 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature