Integrated bioinformatics and experimental analysis of mitochondrial-associated membrane function and mechanism in acute respiratory distress syndrome​​.

ABSTRACT: Acute respiratory distress syndrome (ARDS) is a life-threatening lung condition characterized by severe inflammation, immune dysregulation, and oxidative stress, leading to high mortality (30-40%). This study explores the involvement of MAM-related genes in ARDS pathogenesis through bioinformatics and experimental validation. Publicly available RNA-sequencing data from ARDS and control samples were analyzed to identify differentially expressed genes (DEGs). Functional enrichment, gene set variation analysis (GSVA), and weighted gene co-expression network analysis (WGCNA) were performed to explore pathway alterations and hub gene interactions. Immune cell infiltration analysis was conducted using CIBERSORT. Candidate MAM-related genes were validated in a Poly I: C-induced ARDS mouse model a

SUBMITTER: Zhou Y 

PROVIDER: S-EPMC12241502 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature