Lysozyme-Imprinted Surface Plasmon Resonance Chips Decorated with Gold Nanoparticles for Lysozyme Detection.

ABSTRACT: In this study, lysozyme-imprinted (Lyz-AuNP-MIP) and nonimprinted (AuNP-NIP) surface plasmon resonance (SPR) sensors modified with gold nanoparticles (AuNPs) were prepared for signal amplification and real-time determination of lysozymes from both aqueous lysozymes and artificial plasma, artificial urine, and artificial tear solutions. Furthermore, lysozyme-imprinted (Lyz-MIP) SPR sensors without AuNPs were prepared to study the signal enhancing effect of the AuNPs. The surface morphology of all prepared SPR sensors was characterized by atomic force microscopy and contact angle measurements. After the characterization study, kinetic studies were carried out using lysozyme solutions (pH 7.4 phosphate buffer), artificial plasma, artificial urine, and artificial tear prepared from 0.01-500 μg

SUBMITTER: Eris S 

PROVIDER: S-EPMC12242623 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature