A general transition metal binding aptamer following the Irving-Williams series.

ABSTRACT: Using both Co²⁺ and Ni²⁺ as target metal ions, aptamer selections were carried out by immobilization of a DNA library via hybridization. A set of aptamers were obtained although they all showed binding to both metal ions. The Co-1 aptamer was highly enriched in both selections and it is a general metal binding aptamer with binding affinity following the Irving-Williams series: Mn²⁺ < Fe²⁺ < Co²⁺ < Ni²⁺ < Cu²⁺ > Zn²⁺. Using NMR spectroscopy, Co-1 forms new base pairs with a relatively large structural change upon binding to Zn²⁺, whereas a previously reported Zn-1 aptamer shows less conformational change and rigidifies upon Zn²⁺ binding. The induced-fitting of the Co-1 aptame

SUBMITTER: Wang J 

PROVIDER: S-EPMC12242838 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature