Amniotic membrane-encapsulated chitosan-lecithin nanoparticles promote the regenerative potential of mesenchymal stromal cells and fibroblasts.

ABSTRACT: The human amniotic membrane (hAM) is a promising therapeutic source in regenerative medicine due to its rich content of growth factors and bioactive molecules. Its clinical application, however, has remained limited by challenges in controlled delivery and compositional complexity. To address these issues, we developed a nanoparticle-based formulation (NF-hAM) to encapsulate hAM proteins and enhance their targeted delivery to mesenchymal stem cells (MSCs) and fibroblasts. NF-hAM was synthesized using soy lecithin and chitosan, forming spherical nanoparticles with well-defined morphology. Physicochemical characterization confirmed successful protein encapsulation and nanoparticle stability. In vitro studies demonstrated that NF-hAM was biocompatible and significantly promoted the pro

SUBMITTER: Abou-Shanab AM 

PROVIDER: S-EPMC12243162 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature