Gut microbiota dysbiosis exacerbates heart failure by the LPS-TLR4/NF-κB signalling axis: mechanistic insights and therapeutic potential of TLR4 inhibition.

ABSTRACT:

Objective

This study aimed to investigate the associations between gut microbiota dysbiosis and alterations in cardiac function and to elucidate the underlying molecular mechanisms involved.

Methods

Eighteen rats were divided into a control group (n = 6), a heart failure (HF) group (n = 6), and a TAK-242 intervention group (n = 6). Cardiac function was assessed using small-animal echocardiography. Serum levels of brain natriuretic peptide (BNP) and inflammatory cytokines were measured by ELISA. Western blotting was used to detect phosphorylated p65 (P-p65) protein expression in myocardial tissue, and 16 S rRNA sequencing was performed to analyse the composition of the faecal gut microbiota.

Results

Compared with the control group, the heart failure group presented sig

SUBMITTER: Zhang C 

PROVIDER: S-EPMC12243407 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature