Binding of rainbow trout sperm to egg is mediated by strong carbohydrate-to-carbohydrate interaction between (KDN)GM3 (deaminated neuraminyl ganglioside) and Gg3-like epitope.

ABSTRACT: KDNalpha2-->3Galbeta4Glcbeta1Cer [(KDN)GM3] is a major (approximately 90%) component of total gangliosides found in sperm of rainbow trout (Oncorhynchus mykiss) and was shown to be present prominently at the sperm head by immunochemical staining with its specific mAb kdn3G. Liposomes containing (KDN)GM3 adhere specifically to GalNAcbeta4Galbeta4Glcbeta1Cer (Gg3Cer)-coated plastic plates. Interaction between (KDN)GM3 and Gg3Cer was much stronger than that previously observed between Neu5Acalpha2-->3Galbeta4Glcbeta1Cer and Gg3Cer. (KDN)GM3-Gg3Cer interaction did not require the presence of Ca2+ and Mg2+, but was enhanced in the presence of Mn2+. Fresh trout sperm adhered specifically to Gg3Cer-coated plates under physiological conditions, and the binding was inhibited by pretreatment of sper

SUBMITTER: Yu S 

PROVIDER: S-EPMC122437 | biostudies-literature | 2002 Mar

REPOSITORIES: biostudies-literature