Musculoskeletal organoids-on-chip uncover muscle-bone communication under intermittent hypoxia.

ABSTRACT: Muscle and bone have intimate biochemical associations spatiotemporally. Yet, the muscle-bone dynamic alterations under intermittent hypoxia (IH) remain unclear, primarily due to the lack of suitable microphysiological models. Herein, we developed a novel musculoskeletal organoids-on-chip (MSK OoC), advancing an integrated study of muscle-bone biochemical communication and personalized interventional strategies. Within this MSK OoC, muscle organoids (MOs) replicate in vivo micro-architecture, while bone organoids mimic both the formation and remodeling processes. Utilizing MSK OoC, we discovered that IH-induced muscle pathology suppressed osteogenesis but stimulated osteoclastogenesis. The mitochondria protein Sirt3 in muscle played a pivotal role in regulating bone metabolism via m

SUBMITTER: Tong X 

PROVIDER: S-EPMC12243851 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature