Analysis of the ultraviolet B response in primary human keratinocytes using oligonucleotide microarrays.

ABSTRACT: UV radiation is the most important environmental skin aggressor, causing cancer and other problems. This paper reports the use of oligonucleotide microarray technology to determine changes in gene expression in human keratinocytes after UVB treatment. Examination of the effects of different doses at different times after irradiation gave a global picture of the keratinocyte response to this type of insult. Five hundred thirty-nine regulated transcripts were found and organized into nine different clusters depending on behavior patterns. Classification of these genes into 23 functional categories revealed that several biological processes are globally affected by UVB. In addition to confirming a majority up-regulation of the transcripts related to the UV-specific inflammatory and stress res

SUBMITTER: Sesto A 

PROVIDER: S-EPMC122456 | biostudies-literature | 2002 Mar

REPOSITORIES: biostudies-literature