The subcellular topology of the RNAi machinery is multifaceted and reveals adherens junctions as an epithelial hub.

ABSTRACT: The RNA interference (RNAi) machinery is a key cellular mechanism catalyzing biogenesis and function of miRNAs to post-transcriptionally regulate mRNA expression. The RNAi machinery includes a set of protein complexes with subcellular localization traditionally presented in a uniform fashion: the microprocessor processes miRNAs in the nucleus, whereas the DICER and the RNA-induced silencing complex (RISC) further process and enable activity of miRNAs in the cytoplasm. However, several studies have identified subcellular patterns of RNAi components that deviate from this model. We have particularly shown that RNAi complexes associate with the adherens junctions of well-differentiated epithelial cells, through the E-cadherin partner PLEKHA7. To assess the extent of these subcellular topologi

SUBMITTER: Nair-Menon J 

PROVIDER: S-EPMC12246189 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature