Structural and functional characterization of a porcine intestinal microbial ecosystem developed in vitro.

ABSTRACT: The mammalian digestive tract harbors a vast microbial community that has the potential to modulate numerous health-related processes. Multicompartment dynamic gut models have been developed to study microbial communities in a controlled environment. To verify the assumption that the experimental results produced in vitro in a mechanical device would be highly similar to those obtained from an in vivo study, in this study fecal samples from four pigs were inoculated in a simulator of the porcine intestinal microbial ecosystem (SPIME) and cultured until reaching steady state. The composition and structure of the resultant microbial communities, and the metabolites produced were compared with those harvested from the intestine of the same pigs. Taxonomic abundance identification based on sha

SUBMITTER: Liu L 

PROVIDER: S-EPMC12246222 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature