Local microglial activation induced and labeled in the retina in a novel subretinal hemorrhage mouse model.

ABSTRACT: Subretinal hemorrhage (SRH) is caused by the accumulation of blood between the neurosensory retina and the retinal pigment epithelium or between the retinal pigment epithelium and the choroid. SRH often arises from age-related macular degeneration, traumas, and may occur spontaneously caused by other diseases like hypertension and diabetes. Here, we developed a novel technique - co-injection of blood and a dye-coupled tracer protein, Cholera toxin subunit B (CtB) - to better localize and understand the disease and how it can cause microglial activation, inflammation, and partial vision loss. Our results show that microglia are activated in the inner retinal layers in zones adjacent to blood injection. In contrast, the non-affected zone of the injected eye showed no microglial activation. F

SUBMITTER: Balogh B 

PROVIDER: S-EPMC12246455 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature