USP5-Mediated PD-L1 deubiquitination regulates immunotherapy efficacy in melanoma.

ABSTRACT:

Background

The role of post-translational modifications(PTMs) in PD-L1-mediated immune resistance and melanoma progression remains poorly understood.

Methods

We conducted multi-omics analyses and constructed a prognostic model based on PTM-related genes using machine learning to identify key regulators in melanoma. In vitro and in vivo experiments, including cell culture, flow cytometry, and subcutaneous allografts models, were used to investigate USP5's function. Protein-protein interactions were validated using Western blotting and co-immunoprecipitation, while PD-L1 stability and ubiquitination were assessed using cycloheximide (CHX) chase and ubiquitination assays.

Results

USP5 was identified as a key DUB that specifically deubiquitinates K48-linked polyubiquitin

SUBMITTER: Xie J 

PROVIDER: S-EPMC12247207 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature