PSIG plasmids, MoClo-compatible vectors for efficient production of chimeric double-stranded RNAs in Escherichia coli HT115 (DE3) strain.

ABSTRACT:

Background

Spray-induced gene silencing (SIGS) is a promising strategy for controlling plant diseases caused by pests, fungi, and viruses. The method involves spraying on plant surfaces double-stranded RNAs (dsRNAs) that target pathogen genes and inhibit pathogen growth via activation of the RNA interference machinery. Despite its potential, significant challenges remain in the application of SIGS, including producing large quantities of dsRNAs for field applications. While industrial-scale dsRNA production is feasible, most research laboratories still rely on costly and labor-intensive in vitro transcription kits that are difficult to scale up for field trials. Therefore, there is a critical need for highly efficient and scalable methods for producing diverse dsRNAs in research la

SUBMITTER: Wu CF 

PROVIDER: S-EPMC12247436 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature