Practical considerations for birefringence microscopy of myelin structure: Microscope design and tissue processing for effective imaging.

ABSTRACT: Despite the interest in studying and quantifying the structural integrity of myelin in postmortem brain tissue, current methods for high-resolution imaging of myelin with optical microscopy are not sufficient. While imaging methods must have adequate resolution and sensitivity to detect microstructural alterations to myelin that are relevant in aging and neurodegenerative disease, an equally critical aspect is to minimize myelin damage that is induced during tissue processing steps. Birefringence microscopy (BRM) is a powerful technique that leverages the structural anisotropy of myelin to provide detailed, label-free images of myelin at any diffraction-limited optical resolution, while maintaining a simple and low-cost setup. Building on our previous work, we have developed a new BRM syst

SUBMITTER: Blanke N 

PROVIDER: S-EPMC12247593 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature