A Chromosome End Without Terminal Telomere Repeats is Stable for Multiple Cell Divisions.

ABSTRACT: We have formed new short telomeres in Schizosaccharomyces pombe using an inducible nuclease that cuts near telomere repeats in cells that lack, cannot recruit or cannot fully activate telomerase. Sequencing these new telomeres showed that cells can divide at least 4 times with ~30 bp of non-telomeric sequence at the chromosome end in cells lacking telomerase, which contrasts with current models for the roles of terminal single-stranded telomere repeats and the telomere proteins in telomere protection and replication. Cells that cannot recruit or activate telomerase had similar results, with additional rearrangements or telomere repeat addition, respectively.

SUBMITTER: Zhang H 

PROVIDER: S-EPMC12247633 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature