In C57Bl6 Mice, Obesity and Subsequent Weight Loss Negatively Affected the Skeleton and Shifted the Cortical Bone Metabolome.

ABSTRACT: Obesity and calorie restriction each negatively affect skeletal health. Despite the negative effects of weight loss on the skeleton, obese patients are advised to lose weight via calorie restriction. Additionally, obesity and weight loss individually alter both whole-body and local metabolism. Little is known about bone quality and changes to the cortical metabolome following calorie restriction in obese preclinical models. We hypothesized that caloric restriction would worsen bone quality in obese mice by shifting the cortical bone metabolome. To induce obesity, 8-week-old male and female C57BL6/J mice received 60% high-fat diet for 12 weeks. From 20 to 30 weeks of age, mice either remained obese or lost weight through 30% caloric restriction. Control animals received a 10% low-fat diet.

SUBMITTER: Chlebek C 

PROVIDER: S-EPMC12247641 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature