The Evolutionary Landscape of tRNA Modifications in Archaea: Insights from High-Throughput Sequencing.

ABSTRACT: Transfer RNA (tRNA) modifications play essential roles in structure, stability and decoding accuracy, yet the evolutionary dynamics and diversity of these modifications remain incompletely understood. Recent advances in high-throughput sequencing, including Ordered Two-Template Relay sequencing (OTTR-seq), now facilitate systematic, transcriptome-wide detection of tRNA modifications at single-base resolution. Here we employed OTTR-seq to comprehensively profile tRNA modifications across nine archaeal species spanning diverse ecological niches, including thermophiles, methanogens, acidophiles, and halophiles. We identified coordinated and mutually exclusive methylation patterns at acceptor stem position 6 and 67 in hyperthermophiles, as well as unexpected clade-specific co-modifications at

SUBMITTER: Leavitt JS 

PROVIDER: S-EPMC12247713 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature