High-Resolution Spatial Proteomics Characterises Colorectal Cancer Consensus Molecular Subtypes.

ABSTRACT:

Background

Identification of the consensus molecular subtypes (CMS) opened significant potential for understanding the tumor biology and intertumoral heterogeneity of colorectal cancer (CRC). However, molecular subtyping in CRC traditionally relies on bulk transcriptomics, therefore, lacks spatial and single-cell level aspect.

Methods

We constructed tissue microarrays using tumor cores from 222 CRC patients. Arrays were stained and imaged using 54 cell identity and cancer hallmark markers, delivering spatially resolved protein profiles of >2 million cells. RNA sequencing data and CMS classification were also available for these patients. After segmentation of cancer, stromal and immune cells, we investigated intratumoral heterogeneity within CMS subtypes using spatially reso

SUBMITTER: Kisakol B 

PROVIDER: S-EPMC12247837 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature