Acquired CRISPR spacers and rhamnose-glucose polysaccharide defects confer resistance to <i>Streptococcus mutans</i> phage ɸAPCM01.

ABSTRACT: Streptococcus mutans is a major cause of dental caries worldwide. Targeted therapeutic strategies to eradicate S. mutans include oral phage rinses. In this study, we investigated how phage resistance develops in S. mutans. As a model phage, we used ɸAPCM01, which is known to infect a serotype e strain. We isolated and sequenced the genomes of 15 spontaneous resistant mutants and found that 10 had acquired novel CRISPR spacers targeting the phage, with a total of 18 new spacers identified. Additionally, eight strains contained mutations in rhamnose-glucose polysaccharide (RGP) biosynthetic genes, three of which also acquired spacers. Only the rgp mutants exhibited defects in phage absorption, supporting the role of these cell surface glycans as the phage receptor

SUBMITTER: Wall LA 

PROVIDER: S-EPMC12247853 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature