Proteomic profiling of primary cilia in the developing brain uncovers new regulators of cortical development.

ABSTRACT: In the developing brain, the primary cilia of radial glial cells extend from the surface of the lateral ventricle, serving as the signaling hub to integrate environmental cues critical for brain formation. Dysfunctions in ciliary proteins contribute to a wide range of brain structural abnormalities in ciliopathies, yet identifying the bona fide ciliary protein components in the brain remains a significant challenge. Here, using proximity labeling and quantitative proteomics, we systematically charted proteins localized to the cilia of radial glial cells in the dorsal and ventral regions of the embryonic brain. From this dataset, we identified cohorts of new molecules intrinsic to the cilia at distinct regions of the developing brain. We validated the cilium localization of several componen

SUBMITTER: Liu X 

PROVIDER: S-EPMC12247902 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature