Spatial and longitudinal tracking of enhancer-AAV vectors that target transgene expression to injured mouse myocardium.

ABSTRACT: Tissue regeneration enhancer elements (TREEs) direct expression of target genes in injured and regenerating tissues. Additionally, TREEs of zebrafish origin were shown to direct the expression of transgenes in border zone regions after cardiac injury when packaged into recombinant AAV vectors and introduced into mice. Future implementation of TREEs into AAV-based vectors as research tools and potential gene therapy modalities will require a more detailed understanding of expression dynamics and potential off-target effects. Here, we applied in vivo bioluminescent imaging to mice systemically injected with AAV vectors containing different combinations of capsids, enhancers, and timing of delivery. Longitudinal tracking of TREE-based expression over time revealed distinct amplitudes and dura

SUBMITTER: Wolfson DW 

PROVIDER: S-EPMC12247933 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature