Surface morphometrics reveals local membrane thickness variation in organellar subcompartments.

ABSTRACT: Lipid bilayers form the basis of organellar architecture, structure, and compartmentalization in the cell. Decades of biophysical, biochemical, and imaging studies on purified or in vitro reconstituted liposomes have shown that variations in lipid composition influence the physical properties of membranes, such as thickness and curvature. However, similar studies characterizing these membrane properties within the native cellular context have remained technically challenging. Recent advancements in cellular cryo-electron tomography (cryo-ET) imaging enable high-resolution, three-dimensional views of native organellar membrane architecture preserved in near-native conditions. We previously developed a 'Surface Morphometrics' pipeline that generates surface mesh reconstructions to mod

SUBMITTER: Medina M 

PROVIDER: S-EPMC12247969 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature