Impact of Acute Endurance Exercise on Alternative Splicing in Skeletal Muscle.

ABSTRACT:

Purpose

Alternative splicing (AS) is a highly conserved post-transcriptional mechanism, generating mRNA variants to diversify the proteome. Acute endurance exercise appears to transiently perturb AS in skeletal muscle, but transcriptome-wide responses are not well-defined. We aimed to better understand differential AS (DAS) and differential isoform expression (DIE) in skeletal muscle by comparing short-read (SRS) and long-read RNA sequencing (LRS) data.

Methods

Publicly accessible SRS of clinical exercise studies were extracted from the Gene Expression Omnibus. Oxford Nanopore LRS was performed on mouse gastrocnemius before and following treadmill exercise (30m running, n=5 mice/group, 20 total, 10 weeks old). Differential gene expression (DGE) and DIE were analyzed a

SUBMITTER: Ahn A 

PROVIDER: S-EPMC12248044 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature