Regulation of Transcriptional Bursting and Spatial Patterning in Early <i>Drosophila</i> Embryo Development.

ABSTRACT: Nascent RNA synthesis often occurs in periods of high transcriptional activity, interspersed with basal or no activity periods. This phenomenon, known as transcriptional bursting, drives high intercellular variability in gene expression levels. How do key patterning genes in early Drosophila melanogaster embryos overcome this variability to establish precise spatial patterns for tissue development? To address this question, we study single cell transcriptional activity from MS2-based live imaging data of four transgenic constructs (rho, Kr, sna shadow, sna proximal enhancers) and the endogenous eve gene. We developed an algorithm to infer promoter states in hundreds of cells within the embryo using transcriptional activity data. Results show that while mean tran

SUBMITTER: Nieto C 

PROVIDER: S-EPMC12248051 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature