Exploring OR2H1-Mediated Sperm Chemotaxis: Development and Application of a Novel Microfluidic Device.

ABSTRACT: Microfluidic platforms have emerged as critical technologies for exploring sperm chemotaxis, providing precise gradient control, and facilitating in-depth behavioral assessment. We designed a novel, user-friendly microfluidic device that is optimized for human sperm morphology and motility. The device was validated using two well-established sperm chemoattractants, progesterone and bourgeonal, demonstrating its reliability and reproducibility. Given the key role of olfactory receptors (ORs) in mediating sperm chemotaxis, the newly developed device was employed to identify additional receptors that may contribute to sperm behavior. Using the Atlas database, we identified OR2H1 as a candidate receptor. It is enriched in testis-derived cells, particularly in early and late spermatids, and it

SUBMITTER: Di Nicuolo F 

PROVIDER: S-EPMC12248556 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature