Systematic Investigations of the Huperzine A-Producing Endophytic Fungi of <i>Huperzia serrata</i> in China and Fermentation Optimization Using OSMAC Strategy.

ABSTRACT: Huperzine A (HupA) can alleviate Alzheimer's disease due to its reversible inhibition of acetylcholinesterase (AChE). The chemical synthesis and plant extraction of HupA is plagued by route complexity and resource scarcity, respectively. Although some endophytic fungi from Huperzia serrata can independently biosynthesize HupA, their yields are trivial. After a comprehensive investigation of HupA-producing H. serrata across China, we focused on the endophytic fungi from Hunan and Hubei provinces, which demonstrated high-level HupA. Morphological characteristics and internal transcriptional sequence (ITS) analysis revealed their diversity. Among the four HupA-producing endophytic fungi, Colletotrichum kahawae is the best-performing and was thus subjected to fermentation

SUBMITTER: Li W 

PROVIDER: S-EPMC12251189 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature