Modifying the potato tuber storage protein patatin targeting improved thermal stability.

ABSTRACT:

Main conclusion

Gene editing of the patatin gene cluster using a single-guide RNA sequence consistently modifies over 10% of the targeted genes in modified individuals. Patatins have gained recent attention, as a group of highly nutritious proteins with excellent functional properties. Some techniques have been suggested for industrial-scale patatin purification, mostly as a by-product from potato starch processing. The purification process has proved to be a challenge due to the low thermostability of patatins, especially under acidic conditions. One strategy to make patatin more accessible for extraction would be to stabilize the protein structure through the introduction of point mutations. Here, we show that the tuber expression of patatin genes is dominated by a few genes from

SUBMITTER: Friberg M 

PROVIDER: S-EPMC12254056 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature