Hyaluronan Directs Alveolar Type II Cell Response to Acute Ozone Exposure in Mice.

ABSTRACT: Becoming more frequent because of climate change, ozone (O₃) exposures can cause lung injury. Alveolar type 2 (AT2) cells and hyaluronan (HA), a matrix component, are critical to repairing lung injury and restoring homeostasis. Here, we define the impact of HA on AT2 cells after acute O₃ exposure. C57BL/6J mice were exposed to filtered air or O₃ (2 ppm) for 3 hours. HA was measured in BAL and lung tissue; HAS (HA synthase) 1, 2, and 3 and HYAL (hyaluronidase) 1, 2, and 3 mRNA were measured in lung tissue and BAL cells. At 48 to 72 hours after O₃ exposure, HA increased in BAL fluid by ELISA and lung tissue by immunohistochemistry, with new HA deposition localized to the alveolar ducts. This was associated with increased whole-lung HAS2 mRNA expres

SUBMITTER: Vose A 

PROVIDER: S-EPMC12254692 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature