Unveiling nonribosomal peptide synthetases from the ergot fungus <i>Claviceps purpurea</i> involved in the formation of diverse ergopeptines.

ABSTRACT: Ergopeptines or their derivatives are widely used for treating neurodegenerative and cerebrovascular diseases. The nonribosomal peptide synthetase-d-lysergyl peptide synthetase A (LPSA) determines ergopeptine formation but the detailed mechanism remains to be elucidated. Here, we characterized two LPSAs from Claviceps purpurea Cp-1 strain through heterologous expression in Aspergillus nidulans feeding with d-lysergic acid. We proved that Cp-LPSA1 catalyzed the formation of ergocornine, α-ergocryptine, and β-ergocryptine, precisely controlled by the substrate specificity of its three modules. Cp-LPSA2 was initially inactive but could be restored to catalyze α-ergosine formation. Using this platform, we validated that P1-LPSA1 and P1-LPSA2 from the reported

SUBMITTER: Chen JJ 

PROVIDER: S-EPMC12254699 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature