Validation of microphysiological systems for interpreting patient heterogeneity requires robust reproducibility analytics and experimental metadata.

ABSTRACT: Multi-cell-type, 3D microphysiological systems (MPS) that recapitulate normal organ/organ system functions and the progression of diseases are being applied in drug discovery and development programs to enable precision medicine. A critical step for this application is to demonstrate the reproducibility of the MPS and its ability to identify biologic/clinical heterogeneity from experimental variability, which requires capturing detailed metadata associated with MPS studies as well as a strong analytical approach for assessing reproducibility. Detailed metadata ensure that identical study parameters are being compared when evaluating reproducibility. We have developed the Pittsburgh reproducibility protocol (PReP), which uses a set of common statistical metrics, the coefficient of variation

SUBMITTER: Miedel MT 

PROVIDER: S-EPMC12256941 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature