Single-cell multiomic analysis reveals methylome and transcriptome deviations following oocyte maturation in vitro.

ABSTRACT:

In brief

In vitro maturation is an essential tool in reproductive technologies, though its impact on oocyte quality remains a concern. This study shows that in vitro maturation alters gene expression and DNA methylation in bovine oocytes compared to in vivo matured oocytes, potentially compromising oocyte quality and developmental competence.

Abstract

In vitro maturation of oocytes is a routine step in assisted reproduction but is associated with lower embryo development rates compared to oocyte maturation in vivo. We analyzed the genomic profiles of oocytes from the same cow, either matured in vivo or in vitro, using single-cell methylome and transcriptome sequencing, along with transcriptome analysis of corresponding cumulus cells. Both the transcriptome and methylome of t

SUBMITTER: Benedetti C 

PROVIDER: S-EPMC12257493 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature