The genomic region matters when synthesizing dsRNA for plant virus suppression via RNAi.

ABSTRACT: Plant viruses are one of the most economically important plant pathogen groups in the world, and there is no viricide available for their control. Therefore, RNA interference (RNAi)-based crop protection has become a promising strategy for the control of viral plant pathogens in agricultural systems. Herein, we aimed to test the hypothesis that exogenously applied dsRNA molecules derived from different viral genomic regions induce different levels of viral suppression by RNAi in plants. We also evaluated the fate and movement of the dsRNA molecules inside tobacco plants. We synthesized dsRNAs from three potato virus Y (PVY) cistrons, helper component-protease (HC-Pro), nuclear inclusion protein b (NIb), and coat protein (CP), and applied them to tobacco leaves to test our hypothesis. Our r

SUBMITTER: Barroso K 

PROVIDER: S-EPMC12257704 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature