Observing intersubunit dynamics in single yeast ribosomes.

ABSTRACT: Translation is accompanied by rotation of the small and large ribosomal subunits relative to each other. Here, we use single-molecule Förster resonance energy transfer between fluorophores introduced into ribosomal proteins uS15 and eL30 to follow intersubunit dynamics of Saccharomyces cerevisiae ribosomes. Similar to their bacterial counterparts, yeast ribosomes are observed to sample two predominant FRET states corresponding to the nonrotated (NR) and rotated (R) conformations. Our data yield further evidence that intersubunit rotation is coupled to tRNA transitions between classical and hybrid binding states. The elongation cycle, which comprises tRNA binding, peptide transfer, and mRNA-tRNA translocation, is accompanied by switching from NR to R and then back to the NR conformat

SUBMITTER: Das A 

PROVIDER: S-EPMC12258932 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature